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Volume 101, Issue 2
9 July 2012
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Research Article| July 13 2012
Surendra K. Yadav;
Surendra K. Yadav a)
1
Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory
, Dr. K.S. Krishnan Marg, New Delhi 110012,
India
2
Department of Physics, Motilal Nehru National Institute of Technology
, Allahabad, Allahabad 211004,
India
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Jay Singh;
Jay Singh a)
1
Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory
, Dr. K.S. Krishnan Marg, New Delhi 110012,
India
3
Department of BIN Fusion Technology and Department of Polymer-Nano Science and Technology, Chonbuk National University
, Jeonju, Jeonbuk 561-756,
Korea
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Ved Varun Agrawal;
Ved Varun Agrawal
1
Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory
, Dr. K.S. Krishnan Marg, New Delhi 110012,
India
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B. D. Malhotra
B. D. Malhotra b)
4
Department of Biotechnology, Delhi Technological University
, Shahbad Daulatpur, Delhi 110042,
India
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Author & Article Information
a)
S. K. Yadav and J. Singh contributed equally to this work.
b)
Author to whom correspondence should be addressed. Electronic mail: bansi.malhotra@gmail.com.
Appl. Phys. Lett. 101, 023703 (2012)
Article history
Received:
May 15 2012
Accepted:
June 22 2012
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Citation
Surendra K. Yadav, Jay Singh, Ved Varun Agrawal, B. D. Malhotra; Nanostructured nickel oxide film for application to fish freshness biosensor. Appl. Phys. Lett. 9 July 2012; 101 (2): 023703. https://doi.org/10.1063/1.4736578
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Xanthine oxidase (XOx) has been physisorbed onto nanostructured nickel oxide (n-NiO, ∼41 nm), electrophoretically deposited onto indium-tin-oxide (ITO) glass substrate using poly-ethylene glycol via co-precipitation. The electrochemical response studies of the XOx/n-NiO/ITO bioelectrode show linear response in the xanthine concentration range as 10–200 μM (R2 = 0.9989), sensitivity as 0.6214 μA/μM cm2, detection limit as 2.989 μM, standard deviation as 0.6192 μA, response time of 15 s with the stability of 100 days. The observed low value (0.0306 mM) Michaelis–Menten constant (Km) indicates high affinity of XOx towards xanthine.
Topics
Glass, Scanning electron microscopy, Thin films, Transmission electron microscopy, Nanoparticle, Co-precipitation, Physisorption, Enzymes, Biosensors, Regression analysis
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See supplementary material at http://dx.doi.org/10.1063/1.4736578 for Scheme, Hall–Williamson plot, and comparison table, and the results of FTIR, SEM, contact angle measurement, EIS, scan rate, response time, stability curve, and real sample analysis.
© 2012 American Institute of Physics.
2012
American Institute of Physics
Supplementary Material
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